Regulatory

Part:BBa_K352002:Experience

Designed by: Ozkan IS   Group: iGEM10_METU_Turkey   (2010-10-02)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K352002

The 2015 LASATX iGEM team used this part in our fragrance-producing carbon monoxide sensor project. More information can be found on our Experiment page: http://2015.igem.org/Team:LASATX/Description.

User Reviews

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LASATX 2015

We placed a reporter gene, sfGFP, downstream of the pCoof promoter in the pCDFDuet-1 plasmid. We transformed this plasmid along with another plasmid containing a constitutively produced transcription activator CooA into BL21(DE3) cells. We used cells without the modified plasmid as our negative control, and cells containing plasmids with T7-regulated sfGFP as our positive control. We tested the constructs at 0, 50, 100, 200 and 500 uM CORM-2 (carbon monoxide releasing molecule). pCoof regulated sfGFP did not show a significant correlation between gene expression and CO presence. However, other factors (listed in link below) may have influenced our results.

More details here: http://2015.igem.org/Team:LASATX/Design.

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