Regulatory

Part:BBa_K352002:Experience

Designed by: Ozkan IS   Group: iGEM10_METU_Turkey   (2010-10-02)


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Please enter how you used this part and how it worked out.

Applications of BBa_K352002

The 2015 LASATX iGEM team used this part in our fragrance-producing carbon monoxide sensor project. More information can be found on our Experiment page: http://2015.igem.org/Team:LASATX/Description.

User Reviews

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LASATX 2015

We placed a reporter gene, sfGFP, downstream of the pCoof promoter in the pCDFDuet-1 plasmid. We transformed this plasmid along with another plasmid containing a constitutively produced transcription activator CooA into BL21(DE3) cells. We used cells without the modified plasmid as our negative control, and cells containing plasmids with T7-regulated sfGFP as our positive control. We tested the constructs at 0, 50, 100, 200 and 500 uM CORM-2 (carbon monoxide releasing molecule). pCoof regulated sfGFP did not show a significant correlation between gene expression and CO presence. However, other factors (listed in link below) may have influenced our results.

More details here: http://2015.igem.org/Team:LASATX/Design.

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